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Fusion primers with dual-indexed PCR adapters were used to generate the construct library, and short fragments were removed with AMPure beads

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In other words, high-quality males would have more robust immune function were it not for the immunosuppressive effects of elevated testosterone.) This previous work suggests that although testosterone is a key mediator of investment in males sexual development and mating behavior [1012], it may not be directly responsible for decrements in immunity that occur in the context of elevated reproductive effort